Mosimane called for the introduction of VAR in all of the continental competition's fixtures after questioning several decisions by the officials in Sunday's defeat.
"Of course, we are very sad about the result," Mosimane said post-match.
"It's a match that I didn't want to lose but these things happen in football.
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"I can't blame the team. We had many opportunities to score. I always knew that if we had any threat, it will come from counter-attacks.
"I congratulate Mamelodi Sundowns on winning today's game. I think we have to qualify through the hardest way. We have to try and win in South Africa. We can do that.
Mosimane also felt VAR could have helped with Kennedy Mweene appearing to handle the ball outside the box in the second half.
"I will always ask CAF to use VAR throughout the whole competition. The opponent's goalkeeper used his hand to save the ball from outside of the box. Also, I believe Tau was onside when he scored," he continued.
"However, we don't blame the absence of VAR for the loss. We had many opportunities that we should've scored from."
Sundowns lead Group A with seven points after three games.
While Mosimane's side has it all to do to qualify for the group stages as they sit in third place with just one point from two matches played.
Sundowns and Al Ahly next face off in the reverse fixture in South Africa on Friday, 11 March.