The Sea Robbers went down 3-1 to the Egyptian giants in the 2013 Champions League Final.
Eric Tinkler's men will now revive hostilities with the Red Devils in the semi-finals of the second-tier continental event.
Pirates were paired with Ahly after finishing second to Group B winners Zamalek following a 4-1 loss in Cairo on the weekend.
"The players need no motivation but to motivate themselves," says Sikhosana, who scored in Bucs' Champions League Final triumph in 1995.
"They missed the chance to beat Al-Ahly in the Champions League Final in 2013. This is their second chance. It's up to the players to grab this chance."
'Legs of Thunder' adds: "They know each other. There is no hidden agendas. They know each other's weaknesses and strengths. It's going to be an open game."