The champions named two changes from the Coppa Italia victory over SPAL, with Federico Cheisa introduced and Alvaro Morata introduced for the suspended Dejan Kulusevski.
Chiesa gave justification to his inclusion following 20 minutes after finishing off a ball across space from Morata following a one-two involving Cristiano Ronaldo.
Sampdoria fashioned an opening towards the closing phase of the first half, with a move into the area from Keita Balde picking out Fabio Quagliarella who saw his effort blocked away.
Both sides had chances to finish the half the strongest through a smart attempt from Ronaldo while Antonio Candreva provided the home side's plight from a cross-cum shot.
The Blucerchiati started the second half fired up, eager to turn the tie around with a snap-shot from close-range by Quagliarella blocked from reaching the near post of the net.
Juventus struggled to enjoy openings up top, as the host refused to open up whilst deploying a rigid deep-lying defensive shape that frustrated the visitors.
Andrea Pirlo turned to the substitutes' bench soon before fulltime and called on Federico Bernardeschi for Morata with Adrien Rabiot introduced for Rodrigo Betancur.
It inadvertently culminated with the second a minute in time added on after a square ball from a counter-attack by Juan Cuadrado allowed Aaron Ramsey an easy finish and victory for Juve.